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Quick Details

  • CAS No.: 379669-72-4
  • Other Names: -
  • MF: -
  • EINECS No.: 231-791-2
  • Place of Origin: Guangdong, China (Mainland)
  • Purity: 128ng/ul
  • Classification: High Purity Reagents
  • Brand Name: DSB
  • Model Number: M1021/M1022
  • Color: Blue

Packaging & Delivery

Packaging Details Item No: M1021
Size: 50ug
Item No: M1022
Size: 5x50ug
Pack in vials

Concentration: 128ng/µl  

 

Recommended Loading

2-5 µl/Lane.

 

Concentration

Typical Bands    100 ng/5µl

Other Bands      40 ng/5µl

 

Recommended Electrophoresis Condition

8 cm  5%Agarose Gel, 0.5×TBE, 5 V/cm,  1h.

 

Contents(bp)

60,80,100,120,140,160,180,200,220, 240,260,280,300

 

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

 

Description

20bp Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs. The ladder consists of 13 linear double-stranded fragments .The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

 

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.

 

 

 

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

 

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

 

 

 

 

 

 

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