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Quick Details

  • CAS No.: 100403-24-5
  • Other Names: -
  • MF: -
  • EINECS No.: 231-791-2
  • Place of Origin: Guangdong, China (Mainland)
  • Purity: 144ng/ul
  • Classification: High Purity Reagents
  • Brand Name: DSB
  • Model Number: M1191/M1192
  • Color: Blue

Packaging & Delivery

Packaging Details Item No: M1091
Size: 50ug
Item No: M1092
Size: 5x50ug
Pack in vials

Concentration: 144ng/µl         

 

Recommended Loading

3-5 µl/Lane.

 

Concentration

Typical Bands    100 ng/5µl

Other Bands      40 ng/5µl

 

Recommended Electrophoresis Condition

8 cm 1%Agarose Gel, 1×TAE, 7V/cm, 45min.

 

Contents(bp)

100,200 ,300,400,500,700,1,000, 1,500,2,000,3,000,4,000,5,000, 6,000,8,000,10,000

 

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

 

Description

1 kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments .The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 500bp and 3,000bp are 40ng. The 500bp and 3,000bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

 

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.

 

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