500bp DNA Ladder

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US $0.1-9,999 / Bags | 1 Bag/Bags - (Min. Order)
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Overview
Quick Details
CAS No.:
100403-24-5
Other Names:
-
MF:
-
EINECS No.:
231-791-2
Place of Origin:
Guangdong, China (Mainland)
Purity:
92ng/ul
Classification:
High Purity Reagents
Brand Name:
DSB
Model Number:
M1251,M1252
Color:
Blue
SupplyAbility
SupplyAbility:
100 Bag/Bags per Day -
Packaging & Delivery
Packaging Details
Item no: M1251
Size: 50ug
Item no: M1252
Size: 5x50ug
Pack in vials
Port
Guangzhou

Concentration: 92ng/µl         

 

Recommended Loading

3-5 µl/Lane.

 

Concentration

Typical Bands    100 ng/5µl

Other Bands      40 ng/5µl

 

Recommended Electrophoresis Condition

5 µl/Lane, 8 cm 1%Agarose Gel, 1×TAE, 7V/cm, 45min

 

Contents(bp)

500,1000,1500,2000,2500,3000,3500,4000,4500,5000

 

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

 

Description

500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments .The 2,500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 2,000bp are 40ng. The 2,000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.  

 

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.

 

M1251 500bp ladderM1252 500bp ladder500bp ladder

 

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