DNA Marker 10000

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US $0.1-9,999 / Bag | 1 Bag/Bags (Min. Order)
Supply Ability:
100 Bag/Bags per Day
Port:
Guangzhou
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Overview
Quick Details
CAS No.:
100403-24-5
EINECS No.:
231-791-2
Place of Origin:
Guangdong, China (Mainland)
Purity:
85ng/ul
Classification:
High Purity Reagents
Brand Name:
DSB
Model Number:
M1221/M1222
Color:
Blue
Packaging & Delivery
Packaging Details
Item No: M1221
Size: 50ug
Item No: M1222
Size: 5x50ug
Pack in vials

Concentration: 85ng/µl         

 

Recommended Loading

2-5 µl/Lane.

 

Concentration

Typical Bands     125 ng/5µl

Other Bands      50 ng/5µl

 

Recommended Electrophoresis Condition

5 µl/Lane, 8 cm 1 %Agarose Gel, 1×TAE, 7V/cm,  45min.

 

Contents(bp)

250,500 ,1,000,2,000,4,000,6,000,10,000

 

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

 

Description

DSTM  10000 is ideal for determining the size of double-stranded DNA from 250 to 10,000 base pairs. The ladder consists of 7 linear double-stranded fragments .The 2,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 2,000bp are 50ng. The 2,500bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.

 

 

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.

 

 

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