5 µl/Lane, 8 cm 1.7 %Agarose Gel, 0.5×TBE, 5V/cm, 1h.
1 year at room temperature, for long-time preservation, please storage at -20°C .
Marker 12 is ideal for determining the size of double-stranded DNA from 200 to 2,000 base pairs. The ladder consists of 6 linear double-stranded fragments .The 1,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 1,000bp are 50ng. The 1,000bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.
Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize
it approximately with the amount of DNA in the nearest band of the ladder.
Visualize DNA by staining with ethidium bromide or with SYBR®Green I.